Putative intermediates in the nerve cell differentiation pathway in hydra have properties of multipotent stem cells

We have investigated the properties of nerve cell precursors in hydra by analyzing the differentiation and proliferation capacity of interstitial cells in the peduncle of Hydra oligactis, which is a region of active nerve cell differentiation. Our results indicate that about 50% of the interstitial cells in the peduncle can grow rapidly and also give rise to nematocyte precursors when transplanted into a gastric environment. If these cells were committed nerve cell precursors, one would not expect them to differentiate into nematocytes nor to proliferate apparently without limit. Therefore we conclude that cycling interstitial cells in peduncles are not intermediates in the nerve cell differentiation pathway but are stem cells. The remaining interstitial cells in the peduncle are in G1 and have the properties of committed nerve cell precursors (Holstein and David, 1986). Thus, the interstitial cell population in the peduncle contains both stem cells and noncycling nerve precursors. The presence of stem cells in this region makes it likely that these cells are the immediate targets of signals which give rise to nerve cells

Gravitational-wave detectability of equal-mass black-hole binaries with aligned spins

Binary black-hole systems with spins aligned or anti-aligned to the orbital angular momentum provide the natural ground to start detailed studies of the influence of strong-field spin effects on gravitational wave observations of coalescing binaries. Furthermore, such systems may be the preferred end-state of the inspiral of generic supermassive binary black-hole systems. In view of this, we have computed the inspiral and merger of a large set of binary systems of equal-mass black holes with spins parallel to the orbital angular momentum but otherwise arbitrary. Our attention is particularly focused on the gravitational-wave emission so as to quantify how much spin effects contribute to the signal-to-noise ratio, to the horizon distances, and to the relative event rates for the representative ranges in masses and detectors. As expected, the signal-to-noise ratio increases with the projection of the total black hole spin in the direction of the orbital momentum. We find that equal-spin binaries with maximum spin aligned with the orbital angular momentum are more than "three times as loud" as the corresponding binaries with anti-aligned spins, thus corresponding to event rates up to 30 times larger. We also consider the waveform mismatch between the different spinning configurations and find that, within our numerical accuracy, binaries with opposite spins S_1=-S_2 cannot be distinguished whereas binaries with spin S_1=S_2 have clearly distinct gravitational-wave emissions. Finally, we derive a simple expression for the energy radiated in gravitational waves and find that the binaries always have efficiencies E_rad/M > 3.6%, which can become as large as E_rad/M = 10% for maximally spinning binaries with spins aligned with the orbital angular momentum.Comment: 18 pages, 11 figures, matches published versio

Dynamical overlap simulations using HMC

We apply the Hybrid Monte Carlo method to the simulation of overlap fermions. We give the fermionic force for the molecular dynamics update. We present early results on a small dynamical chiral ensemble.Comment: Lattice2004(machines), 3 pages; references updated, minor changes to tex

Cell cycle length, cell size, and proliferation rate in hydra stem cells

We have analyzed the cell cycle parameters of interstitial cells in Hydra oligactis. Three subpopulations of cells with short, medium, and long cell cycles were identified. Short-cycle cells are stem cells; medium-cycle cells are precursors to nematocyte differentiation; long-cycle cells are precursors to gamete differentiation. We have also determined the effect of different cell densities on the population doubling time, cell cycle length, and cell size of interstitial cells. Our results indicate that decreasing the interstitial cell density from 0.35 to 0.1 interstitial cells/epithelial cell (1) shortens the population doubling time from 4 to 1.8 days, (2) increases the [3H]thymidine labeling index from 0.5 to 0.75 and shifts the nuclear DNA distribution from G2 to S phase cells, and (3) decreases the length of G2 in stem cells from 6 to 3 hr. The shortened cell cycle is correlated with a significant decrease in the size of interstitial stem cells. Coincident with the shortened cell cycle and increased growth rate there is an increase in stem cell self-renewal and a decrease in stem cell differentiation

Swift observations of the 2006 outburst of the recurrent nova RS Ophiuchi: III. X-ray spectral modelling

Following the Swift X-ray observations of the 2006 outburst of the recurrent nova RS Ophiuchi, we developed hydrodynamical models of mass ejection from which the forward shock velocities were used to estimate the ejecta mass and velocity. In order to further constrain our model parameters, here we present synthetic X-ray spectra from our hydrodynamical calculations which we compare to the Swift data. An extensive set of simulations was carried out to find a model which best fits the spectra up to 100 days after outburst. We find a good fit at high energies but require additional absorption to match the low energy emission. We estimate the ejecta mass to be in the range (2-5) x 10^{-7} solar masses and the ejection velocity to be greater than 6000 km/s (and probably closer to 10,000 km/s). We also find that estimates of shock velocity derived from gas temperatures via standard model fits to the X-ray spectra are much lower than the true shock velocities.Comment: 13 pages, 5 figures, Accepted for publication in Ap

What clients want: a conjoint analysis of precursors to coach selection

This study investigated individuals’ preference structures for workplace coaching providers. Guided by questions about relative weightings of seven important coach(ing) characteristics (i.e., coach work experience/background/gender; coaching training; personal recommendations; client feedback; coaching cost), we carried out a conjoint analysis, using a mixed occupational sample (N = 383). In addition, we conducted linear regression analyses to determine the extent to which coaches’ perceived competence, likeability and trustworthiness might impact on individuals’ decision-making processes. Potential coachees favoured professionally trained coaches with four to ten years’ experience and a similar background to themselves, were female, and charged below average fees. Personal recommendations and existing client feedback further influenced potential consumers’ decision-making. Moreover, perceived competence was highly predictive of potential coach selection

Accurate Realizations of the Ionized Gas in Galaxy Clusters: Calibrating Feedback

Using the full, three-dimensional potential of galaxy cluster halos (drawn from an N-body simulation of the current, most favored cosmology), the distribution of the X-ray emitting gas is found by assuming a polytropic equation of state and hydrostatic equilibrium, with constraints from conservation of energy and pressure balance at the cluster boundary. The resulting properties of the gas for these simulated redshift zero clusters (the temperature distribution, mass-temperature and luminosity-temperature relations, and the gas fraction) are compared with observations in the X-ray of nearby clusters. The observed properties are reproduced only under the assumption that substantial energy injection from non-gravitational sources has occurred. Our model does not specify the source, but star formation and AGN may be capable of providing this energy, which amounts to 3 to 5 x10^{-5} of the rest mass in stars (assuming ten percent of the gas initially in the cluster forms stars). With the method described here it is possible to generate realistic X-ray and Sunyaev-Zel'dovich cluster maps and catalogs from N-body simulations, with the distributions of internal halo properties (and their trends with mass, location, and time) taken into account.Comment: Matches ApJ published version; 30 pages, 7 figure

Reducing the weak lensing noise for the gravitational wave Hubble diagram using the non-Gaussianity of the magnification distribution

Gravitational wave sources are a promising cosmological standard candle because their intrinsic luminosities are determined by fundamental physics (and are insensitive to dust extinction). They are, however, affected by weak lensing magnification due to the gravitational lensing from structures along the line of sight. This lensing is a source of uncertainty in the distance determination, even in the limit of perfect standard candle measurements. It is commonly believed that the uncertainty in the distance to an ensemble of gravitational wave sources is limited by the standard deviation of the lensing magnification distribution divided by the square root of the number of sources. Here we show that by exploiting the non-Gaussian nature of the lensing magnification distribution, we can improve this distance determination, typically by a factor of 2--3; we provide a fitting formula for the effective distance accuracy as a function of redshift for sources where the lensing noise dominates.Comment: matches PRD accepted version (expanded description of the cosmological parameter space + minor changes

A multiwavelength study of the remnant of nova GK Persei

We present new observations of the nebular remnant of the old nova GK Persei 1901, in the optical using the 2m HCT and at low radio frequencies using the GMRT. The evolution of the nova remnant indicates shock interaction with the ambient medium, especially in the southwest quadrant. Application of a simple model for the shock and its evolution to determine the time dependence of the radius of the shell in the southwest quadrant indicates that the shell is now expanding into an ambient medium that has a lower density compared to the density of the ambient medium ahead of the shock in 1987.There are indications of a recent interaction of the nova remnant with the ambient medium in the northeast quadrant also. The nova remnant of GK Per is detected at all the observed radio frequencies and is of similar extent as the optical remnant. Putting together our radio observations with VLA archival data on GK Per from 1997, we obtain three interesting results: 1. The spectrum above 1.4 GHz follows a power law with an index -0.7 and below 1.4 GHz follows a power law with an index ~ -0.85. This could be due to the presence of at least two populations of electrons dominating the global emission at different frequencies. 2. We record an annual secular decrease of 2.1% in the flux density of the nova remnant at 1.4 and 4.9 GHz between 1984 and 1997 which has left the spectral index unchanged at -0.7. No such decrease is observed in the flux densities below 1 GHz. 3. We record an increase in the flux density at 0.33 GHz compared to the previous estimate in 1987. We conclude that the remnant of nova GK Per is similar to supernova remnants and in particular, to the young supernova remnant Cas A.Comment: 10 pages; uses A&A style; figures 1, 2 & 6 are in JPEG format. Accepted for publication in Astronomy & Astrophysics. Full paper including Figures 1, 2 & 6 may be downloaded from http://www.iiap.res.in/personnel/gca/gca.htm

New analytic running coupling in QCD: higher loop levels

The properties of the new analytic running coupling are investigated at the higher loop levels. The expression for this invariant charge, independent of the normalization point, is obtained by invoking the asymptotic freedom condition. It is shown that at any loop level the relevant $\beta$ function has the universal behaviors at small and large values of the invariant charge. Due to this feature the new analytic running coupling possesses the universal asymptotics both in the ultraviolet and infrared regions irrespective of the loop level. The consistency of the model considered with the general definition of the QCD invariant charge is shown.Comment: LaTeX 2.09, 12 pages with 5 EPS figures, uses mpla1.sty; enlarged version is accepted for publication in Mod. Phys. Lett.